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identx 3 3d cell culture chip  (AIM Biotech)

 
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    AIM Biotech identx 3 3d cell culture chip
    Identx 3 3d Cell Culture Chip, supplied by AIM Biotech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/3d+cell+culture+chips+identx+3+chip/identx+3+3d+cell+culture+chip/pm40319918-280-14-21
    Average 90 stars, based on 1 article reviews
    identx 3 3d cell culture chip - by Bioz Stars, 2026-09
    90/100 stars

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    Cell Culture:

    Article Title: Age-related alterations in meningeal immunity drive impaired CNS lymphatic drainage.
    Article Snippet: For the 3D microfluidic cultures, the same media supplemented with vascular endothelial growth factor-c (VEGF-C; R&D Systems), angiopoietin-1 (ANG-1; R&D Systems), and hepatocyte growth factor (HGF; Peprotech) were used at a 50 ng/ml concentration. .. 3D cell culture chips (idenTx 3 Chip; AIM Biotech) were filled with a 1:1 ratio of fibrinogen (cat. no. F8630-1G; Sigma-Aldrich, stock concentration 5 mg/ml in PBS) and thrombin (T4648-1KU; Sigma-Aldrich, 4 U/ml final thrombin solution diluted in basal media) to form the fibrin gel in the central region of the device by incubating the chips for 15 min at 37°C and 5% CO2. .. Then the media channel side that will host the lymphatic cells was coated with human plasma fibronectin (EMD Millipore) at a concentration of 100 μg/ml for 45 min and then washed out.

    Concentration Assay:

    Article Title: Age-related alterations in meningeal immunity drive impaired CNS lymphatic drainage.
    Article Snippet: For the 3D microfluidic cultures, the same media supplemented with vascular endothelial growth factor-c (VEGF-C; R&D Systems), angiopoietin-1 (ANG-1; R&D Systems), and hepatocyte growth factor (HGF; Peprotech) were used at a 50 ng/ml concentration. .. 3D cell culture chips (idenTx 3 Chip; AIM Biotech) were filled with a 1:1 ratio of fibrinogen (cat. no. F8630-1G; Sigma-Aldrich, stock concentration 5 mg/ml in PBS) and thrombin (T4648-1KU; Sigma-Aldrich, 4 U/ml final thrombin solution diluted in basal media) to form the fibrin gel in the central region of the device by incubating the chips for 15 min at 37°C and 5% CO2. .. Then the media channel side that will host the lymphatic cells was coated with human plasma fibronectin (EMD Millipore) at a concentration of 100 μg/ml for 45 min and then washed out.



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